重组人 IGFBP4 蛋白 (His标签)（产品说明）
蛋白名称：Human IGFBP4 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human IGFBP4 (NP_001543.2) precursor (Met 1-Glu 258) with a C-terminal polyhistidine tag was expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to bind human IGF1 (Cat:10598-H24E) in functional ELISA. 2. Measured by its ability to bind human IGF2 (Cat:13032-H24E) in functional ELISA. 3. Measured by its ability to inhibit the biological activity of IGFI or IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 0.04-0.4 μg/mL in the presence of 14 ng/mL human IGFII.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asp 22
蛋白分子量：The secreted recombinant human IGFBP4 comprises 248 amino acids with a predicted molecular mass of 27.4 kDa. As a result of glycosylation, it migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001543.2
蛋白氨基酸序列：Met1-Glu258
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.