图文详情
Interferon alpha 2/IFNA2重组蛋白|Recombinant Human Interferon alpha 2/IFNA2 Protein(His&AVI Tag),Biotinylated(产品说明)
蛋白名称:Human Interferon alpha 2 / IFNA2 Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Human Interferon alpha 2 / IFNA2 (P01563-1) (Met1-Glu188) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:Immobilized Recombinant Human Interferon alpha 2/IFNA2 Protein(His&AVI Tag),Biotinylated (Cat: 13833-H49H-B ) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IFNAR2 / IFNABR Protein (Fc Tag) (Cat: 10359-H02H), the EC50 is 12-40 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Cys 24
蛋白分子量:The recombinant Human Interferon alpha 2 / IFNA2 consisting of 191 amino acids and has a calculated molecular mass of 22.53 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 25.1 kDa and 23.5 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:P01563-1
蛋白氨基酸序列:Met1-Glu188
蛋白标签:C-His-AVI