图文详情
小鼠 ICOS ligand蛋白|ICOS ligand protein|ICOS ligand(Mouse, C-human IgG1-Fc & His)(产品说明)
蛋白名称:ICOS ligand蛋白, ICOS ligand protein
蛋白构建:A DNA sequence encoding the mouse B7-H2 (NP_056605.1) extracellular domain (Met 1-Lys 279) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized human ICOS at 1 μg/ml (100 μl/well) can bind biotinylated mouse B7-H2 Fc chimera with a linear range of 0.125-1.0 μg/ml .
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 47
蛋白分子量:The secreted recombinant mouse B7-H2/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer comprises 481 amino acids with a predicted molecular mass of 54.3 kDa. As a result of glycosylation, it migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_056605.1
蛋白氨基酸序列:Met1-Lys279
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.