ICAM-1/CD54重组蛋白|Recombinant Human ICAM-1 / CD54 Protein (His & Fc Tag)（产品说明）
蛋白名称：Human ICAM-1 / CD54 Protein (His & Fc Tag)
蛋白构建：A DNA sequence encoding the human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10E4 cells/well are added to Recombinant Human ICAM-1/CD54 coated plates (12.5 μg/mL with 100 μL/well), >40% cells will adhere after 1 hour of incubation at 37℃.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 28
蛋白分子量：The recombinant human ICAM1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 700 amino acids and has a predicted molecular mass of 77.4 kDa. As a result of glycosylation, the apparent molecular mass of rh ICAM1/Fc monomer migrates with an apparent molecular mass of 100-110 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000192.2
蛋白氨基酸序列：Met1-Glu480
蛋白标签：C-human IgG1-Fc & His