重组甲型流感 H5N1 HA 蛋白 (AVI & His Tag), Biotinylated（产品说明）
蛋白名称：Influenza A H5N1 (A/Texas/37/2024; A/dairy cow/Texas/24-008749-002-v/2024) Hemagglutinin / HA Protein (AVI & His Tag), Biotinylated(MALS-verified)
蛋白构建：A DNA sequence encoding the Influenza A H5N1 (A/Texas/37/2024; A/dairy cow/Texas/24-008749-002-v/2024) Hemagglutinin / HA (translated amino acids of EPI2419700), termed as HA, was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白活性：Immobilized Recombinant Influenza A H5N1 HA Protein (AVI & His Tag), Biotinylated(Cat: 41036-V27H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-Influenza HA (Research Grade Clone CR9114), the EC50 is 0.4-1.2 ng/mL (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
蛋白分子量：The recombinant Influenza A H5N1 (A/Texas/37/2024; A/dairy cow/Texas/24-008749-002-v/2024) Hemagglutinin / HA (translated amino acids of EPI2419700), termed as HA, comprises 574 amino acids and predicts a molecular mass of 64.86 kDa. As a result of glycosylation, it migrates as an approximately 81.9, 50.1 and 32.7 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：EPI3171488 & EPI3182065