图文详情
重组甲型流感 H1N1Hemagglutinin/HA蛋白(产品说明)
蛋白名称:Influenza A H1N1 (A/Victoria/2570/2019) Hemagglutinin / HA-specific B cell probe (Y108F, His & AVI Tag), Biotinylated (HPLC-verified)
蛋白构建:A DNA sequence encoding the Influenza A virus (A/Victoria/2570/2019 (H1N1)) hemagglutinin (EPI2413494) (with a mutation Tyr108Phe), was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
蛋白活性:Immobilized Anti-Influenza HA (Research Grade Clone CR9114) at 2 μg/mL (100 μL/well) can bind Recombinant Influenza A H1N1 HA (Y108F, His & AVI Tag), Biotinylated (Cat: 41050-V49H1-B) , the EC50 is 1.2-4 ng/mL (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
蛋白分子量:The recombinant Influenza A virus (A/Victoria/2570/2019 (H1N1)) hemagglutinin comprises 576 amino acids and has a predicted molecular mass of 64.18 kDa. It migrates as approximately 77.9 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:EPI2413494