图文详情
重组甲型流感Hemagglutinin/HA蛋白,生物素标记(产品说明)
蛋白名称:Influenza A H3N2 (A/Massachusetts/18/2022; A/Thailand/8/2022) Hemagglutinin / HA-specific B cell probe Protein (His & AVI Tag), Biotinylated(MALS-verified)
蛋白构建:A DNA sequence encoding the Influenza A virus (A/Massachusetts/18/2022; A/Thailand/8/2022 (H3N2)) Hemagglutinin / HA-specific B cell probe (EPI2096148 & EPI2178977) (Met1-Val521, with a mutation Tyr114Phe), termed as HA, was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS.
蛋白活性:Immobilized Anti-HA antibody (Research Grade Clone CR8020) at 2 μg/mL (100 μL/well) can bind Recombinant Influenza A H3N2 HA Protein (Y114F, His Tag), Biotinylated (Cat: 40992-V49H2-B), the EC50 is 7-25 ng/mL (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gln 17
蛋白分子量:The recombinant Influenza A virus ((A/Massachusetts/18/2022; A/Thailand/8/2022) (H3N2))Hemagglutinin / HA comprises 574 amino acids and has a predicted molecular mass of 64.25 kDa. It migrates as an approximately 85.5 kDa band in SDS-PAGE under reducing conditions.The molecular weight of this protein is around 240.3 kDa verified by SEC-MALS.
蛋白NP号:EPI2096148 & EPI2178977
蛋白氨基酸序列:Met1-Val521