GPRC5D重组蛋白|Recombinant Cynomolgus / Rhesus GPRC5D Protein (Nanodisc, His & Flag Tag)（产品说明）
蛋白名称：Cynomolgus / Rhesus GPRC5D Protein (Nanodisc, His & Flag Tag)
蛋白构建：A DNA sequence encoding the Cynomolgus / Rhesus GPRC5D (A0A2K5W6I7) (Met1-Ser268) was expressed and linked by a Fusion Protein, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells
蛋白纯度：≥ 80 % as determined by SDS-PAGE.
蛋白活性：Immobilized Recombinant Rhesus GPRC5D-Nanodisc Protein (His & Flag Tag) (Cat: 91022-C18H-NA) at 5 μg/mL (100 μL/well) can bind Anti-Rhesus GPRC5D Antibody, the EC50 is 17.5-52.5 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant Cynomolgus / Rhesus GPRC5D consists of 286 amino acids and predicts a molecular mass of 32.95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 31.7 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：A0A2K5W6I7
蛋白氨基酸序列：Met1-Ser268
蛋白标签：N-Flag & C-His