图文详情
小鼠 GFR Alpha-1/GFRA1蛋白|GFR Alpha-1/GFRA1 protein|GFR Alpha-1/GFRA1(Mouse, C-His)(产品说明)
蛋白名称:GFR Alpha-1/GFRA1蛋白, GFR Alpha-1/GFRA1 protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Ser 425) of mouse GFRα1 isoform beta (AAB86600.1) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 98 % as determined by SDS-PAGE
蛋白活性:1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-1.5 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF. 2. Measured by its ability to bind with human GDNF (Cat:10561-HNCH) in a functional ELISA.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Asp 25
蛋白分子量:The secreted recombinant mouse GFRα1consists of 412 amino acids and has a calculated molecular mass of 46.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:AAB86600.1
蛋白氨基酸序列:Met1-Ser425
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.