图文详情
GDNF重组蛋白|Recombinant Human GDNF Protein(产品说明)
蛋白名称:Human GDNF Protein
蛋白构建:A DNA sequence encoding the mature form of human GDNF (P39905-2) (Arg 83-Ile 185) was expressed and purified, with additional two aa (Gly& Pro) at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-3 ng/mL in the presence of Recombinant Human GFR alpha‑1/GDNF R alpha‑1 His Chimera.
2. Immobilized Recombinant Human GDNF Protein(Cat:10561-HNCH) at 2 μg/mL (100 μL/well) can bind Recombinant Human GFRA1 / GFRα1 / GDNFRA Protein (His Tag) (Cat:10330-H08H), the EC50 is 15-48 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gly
蛋白分子量:The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号:P39905-2
蛋白氨基酸序列:Arg83-Ile185