重组人FOLR1蛋白（APC定点偶联，His标签）（产品说明）
蛋白名称：Human FOLR1 Protein (Site-Specific APC Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human FOLR1 (NP_057937.1) (Met 1-Met 233) without the pro peptide was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).
表达宿主：HEK293 Cells1.Flow cytometric analysis of anti-FOLR1 CAR expression. 5e5 of anti-FOLR1 CAR-293 cells were stained with 100 μL of 1:50 (2 μL stock solution in 100 μL FACS buffer) of APC-conjugated human FOLR1 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 11241-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested). 2.Binding activity of APC-conjugated FOLR1 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human FOLR1 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 11241-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Arg 25
蛋白分子量：The protein has a predicted molecular mass of 27.9 kDa.
蛋白NP号：NP_057937.1
蛋白氨基酸序列：Met1-Met233