重组人FLT3蛋白（APC定点偶联,T227M,His标签）（产品说明）
蛋白名称：Human FLT3 Protein (Site-Specific APC Conjugation, T227M, His Tag)
蛋白构建：A DNA sequence encoding the human FLT3 (P36888-1, with natural variant Thr 227 Met) (Met 1-Asn 541) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).
表达宿主：HEK293 Cells
蛋白活性：1.Flow cytometric analysis of anti-FLT3 CAR expression. 5e5 of anti-FLT3 CAR-293 cells were stained with 100 μL of 1:100 (1 μL stock solution in 100 μL FACS buffer) of APC-conjugated human FLT3 Protein (Site-Specific APC Conjugation, T227M, His Tag, Cat. No. 10445-H86H-SA ) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested). 2.Binding activity of APC-conjugated FLT3 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human FLT3 Protein (Site-Specific APC Conjugation, T227M, His Tag, Cat. No. 10445-H86H-SA ) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Asn 27
蛋白分子量：The protein has a predicted molecular mass of 61.4 kDa.
蛋白NP号：P36888-1, with natural variant Thr 227 Met
蛋白氨基酸序列：Met1-Asn541