FCGRT & B2M Heterodimer重组蛋白|Recombinant Canine FCGRT & B2M Heterodimer Protein(AVI & His Tag),Biotinylated（产品说明）
蛋白名称：Canine FCGRT & B2M Heterodimer Protein (AVI & His Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Canine FCGRT (XP_038512242.1) (Ala23-Pro298) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding Canine B2M (NP_001271408.1) (Met1-Asn125) was fused , constructed the plasmid 2. The two plasmids were co-expressed and the Canine FCGRT & B2M Heterodimer was purified. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 95 % as determined by SEC-MALS(Routinely tested).
蛋白活性：Biotinylated Recombinant Canine FCGRT & B2M Heterodimer Protein, AVI & His Tag (Cat. No. CT148-D27H-B) captured on NTA chip, can bind Rituximab with an affinity constant of 0.211 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Ala 23 & Val 28
蛋白分子量：The recombinant Canine FCGRT & B2M Heterodimer comprises 400 (302+98) amino acids and has a predicted molecular mass of 45.22 (33.7+11.52） kDa. It migrates as an approximately 36.4 and 13.1 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 48.7 kDa verified by SEC-MALS(Routinely tested).
蛋白NP号：XP_038512242.1 & NP_001271408.1
蛋白氨基酸序列：Ala23-Pro298 & Met1-Asn125