人 CD16a蛋白|CD16a protein|CD16a(Human, C-AVI & His)（产品说明）
蛋白名称：CD16a蛋白, CD16a protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (AAH17865.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin–Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to bind recombinant human IgG1 in a functional ELISA. 2. Measured by its binding ability in a functional ELISA. Immobilized human CD16a-AVI-His (Cat:10389-H27H1-B) at 10 μg/ml (100μl/well) can bind recombinant human IgG1 (Fc) (Cat:10702-HNAC) with a linear range of 0.31-5 μg/ml. 3. Using the Octet RED System, the affinity constant (Kd) of CD16a Protein, Human, Recombinant (176 Val, His & AVI Tag), Biotinylated (Cat. 10389-H27H1-B) bound to Conbercept was 0.1 μM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gly 17
蛋白分子量：The secreted recombinant human CD16a consists of 227 amino acids and predicts a molecular mass of 25.6 KDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 KDa due to glycosylation.
蛋白NP号：AAH17865.1
蛋白氨基酸序列：Met1-Gln208
蛋白标签：C-AVI & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.