图文详情
CD32B/Fcgr2b重组蛋白|Recombinant Rhesus CD32B/Fcgr2b Protein (His & AVI Tag), Biotinylated(产品说明)
蛋白名称:Rhesus CD32B/Fcgr2b Protein (His & AVI Tag), Biotinylated
蛋白构建:A DNA sequence encoding the Rhesus FCGR2B (F7GVR2) (Met1-Pro224) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性:Loaded Recombinant Rhesus CD32B/Fcgr2b Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 91023-K49H-B) on SA Biosensor, can bind Bevacizumab(IgG1) with an affinity constant of 0.98 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 46
蛋白分子量:The recombinant Rhesus FCGR2B consisting of 205 amino acids and has a calculated molecular mass of 23.22 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 31.6 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:F7GVR2
蛋白氨基酸序列:Met1-Pro224
蛋白标签:C-His-AVI