人 CD32B/Fcgr2b蛋白|CD32B/Fcgr2b protein|CD32B/Fcgr2b(Human, C-AVI & His)（产品说明）
蛋白名称：CD32B/Fcgr2b蛋白, CD32B/Fcgr2b protein
蛋白构建：A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b (Cat:10259-H27H-B). The EC50 of Human CD32b (Cat:10259-H27H-B) is 1.6 - 3.7 μg/ml. 2. Using the Octet RED System, the affinity constant (Kd) of CD32B / Fcgr2b Protein, Human, Recombinant (His & AVI Tag), Biotinylated (Cat. 10259-H27H-B) bound to Avastin was 0.02 μM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ala 46
蛋白分子量：The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35 kDa protein due to glycosylation.
蛋白NP号：NP_001002274.1
蛋白氨基酸序列：Ala46-Ile224
蛋白标签：C-AVI & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.