重组人 FAS / CD95 / APO-1 / TNFRSF6 蛋白 (Fc标签)（产品说明）
蛋白名称：Human FAS / CD95 / APO-1 / TNFRSF6 Protein (Fc Tag)
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Glu 173) of human Fas antigen (NP_000034.1) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.3-2 μg/mL in the presence of recombinant human Fas ligand.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 26
蛋白分子量：The recombinant human Fas/Fc chimera is a disulfide-linked homodimeric protein generated after removal of the signal peptide. The reduced monomer consists of 386 amino acids and has a predicted molecular mass of 43.4 kDa. In SDS-PAGE under reducing conditions, the monomer migrates as an approximately 55-60 kDa protein due to glycosylation.
蛋白NP号：NP_000034.1
蛋白氨基酸序列：Met1-Glu173
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.