图文详情
重组人 Coagulation Factor XI / FXI / F11 蛋白 (His标签)(产品说明)
蛋白名称:Human Coagulation Factor XI / FXI / F11 Protein (His Tag)
蛋白构建:A DNA sequence encoding the human F11 (NP_000119.1) precursor (Met 1-Val 625) with a carboxy-terminal polyhistidine tag was expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 19
蛋白分子量:The secreted recombinant human F11 consists of 618 amino acids with the predicted molecular mass of 69.5 kDa. As a result of glycosylation, rhCTSS migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_000119.1
蛋白氨基酸序列:Met1-Val625
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.