规格/价格
20 µg：3870
100 µg：8940

Her2/ERBB2 Protein, Cynomolgus, Recombinant (ECD, His & AVI Tag), HPLC-verified: 产品信息

• 纯度：≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• 内毒素：< 1.0 EU per μg protein as determined by the LAL method.
• 生物素标记率：0.7-1 as determined by the HABA assay.
• 生物活性：Testing in progress
• 蛋白构建：A DNA sequence encoding the Cynomolgus ERBB2 (XP_001090430.1) (Met1-Thr652) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
• 表达宿主：HEK293 Cells
• 种属：Cynomolgus
• 预测 N 端：Thr 23
• 分子量：The recombinant Cynomolgus ERBB2 consists of 656 amino acids and predicts a molecular mass of 72.63 kDa. It migrates as an approximately 107.07 kDa band in SDS-PAGE under reducing conditions.
• 缓冲液

1. Lyophilized from sterile PBS, pH 7.4.
2. Please contact us for any concerns or special requirements.
3. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
4. Please refer to the specific buffer information in the hard copy of CoA.
5. 运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
6. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

• 稳定性 & 储存条件

1. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
2. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

• 复溶：A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.

Her2/ERBB2 背景信息
Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

全称
erb-b2 receptor tyrosine kinase 2
研究领域
Cancer Drug Targets
Receptor Tyrosine Kinases (RTKs)