EPHA5重组蛋白|Recombinant Human EPHA5 Protein (ECD, Fc Tag)（产品说明）
蛋白名称：Human EPHA5 Protein (ECD, Fc Tag)
蛋白构建：A DNA sequence encoding the Human EPHA5 (P54756-1) (Met1-Pro573) was expressed with the fused Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：1.Immobilized Recombinant Human EPHA5 Protein (ECD, Fc Tag) (Cat: 29795-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human Ephrin-A3 / EFNA3 Protein (His Tag) (Cat: 10188-H08H) , the EC50 is 250-750 ng/mL. 2.Loaded Recombinant Human EPHA5 Protein, Fc Tag (Cat. No. 29795-H02H) on proA Biosensor, can bind Recombinant Human Ephrin A3 Protein, His Tag (Cat. No. 10188-H08H) with an affinity constant of 0.446 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Pro 25
蛋白分子量：The recombinant Human EPHA5 consists of 787 amino acids and predicts a molecular mass of 87.53 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 109.5 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：P54756-1
蛋白氨基酸序列：Met1-Pro573