EpCAM/TROP1重组蛋白|Recombinant Human EpCAM Protein (ECD, His Tag, Site-Specific AF488 Conjugation)（产品说明）
蛋白名称：Human EpCAM / TROP1 Protein (ECD, His Tag, Site-Specific AF 488 Conjugation)
蛋白构建：A DNA sequence encoding the Human EpCAM (NP_002345.1) (Met1-Lys265) was expressed with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with AF 488 (Excitation Max. = 495 nm, Emission Max. = 519 nm).
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE.
蛋白活性：1.Flow cytometric analysis of anti-EpCAM CAR expression. 293 cells were lentivirally transduced with anti-EpCAM CAR. Flow cytometric analysis was performed with a negative control protein and AF 488-conjugated human EpCAM protein (site-specific AF 488 conjugation, ECD, his tag, Cat. No. 10694-H86H-SD), respectively. Non-transduced 293 cells were used as a control. 2.Binding activity of PE-labeled EpCAM protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 488-conjugated human EpCAM protein (ECD, his tag, site-specific AF488 conjugation, Cat. No. 10694-H86H-SD) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 24
蛋白分子量：The recombinant Human EpCAM consists of AA Met1-Lys265 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 30.9 kDa.
蛋白NP号：NP_002345.1
蛋白氨基酸序列：Met1-Lys265
蛋白标签：C-His