图文详情
人 Ephrin B2蛋白|Ephrin B2 protein|Ephrin B2(Human, C-human IgG1-Fc & His)(产品说明)
蛋白名称:Ephrin B2蛋白, Ephrin B2 protein
蛋白构建:A DNA sequence encoding the human EFNB2 (NP_004084.1) extracellular domain (Met 1-Ala 229) was was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized human EphB4-his at 2 μg/ml (100 μl/well) can bind human EphrinB2 Fc chimera with a linear range of 1-25 ng/ml . Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 Fc chimera with a linear range of 1-125 ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ile 28
蛋白分子量:The recombinant human EFNB2/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 450 amino acids and predicts a molecular mass of 50.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EFNB2/Fc monomer is approximately 62 kDa due to glycosylation.
蛋白NP号:NP_004084.1
蛋白氨基酸序列:Met1-Ala229
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.