重组人 GM-CSF / CSF2 蛋白 (Fc标签)（产品说明）
蛋白名称：Human GM-CSF / CSF2 Protein (Fc Tag)
蛋白构建：A DNA sequence encoding the mature form of human GM-CSF (NP_000749.2) (Ala 18-Glu 144) was fused with the Fc region of human IgG1 at the N-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 97 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized CD131 at 10 μg/ml (100 μl/well) can bind recombinant human GM-CSF / Fc with a linear range of 0.032-4 μg/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-5 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 20
蛋白分子量：The recombinant human GM-CSF/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 364 amino acids and has a predicted molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh GM-CSF/Fc monomer is approximately 48-55 kDa due to glycosylation.
蛋白NP号：NP_000749.2
蛋白氨基酸序列：Ala18-Glu144
蛋白标签：N-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.