Claudin-4/CLDN4重组蛋白|Recombinant Human Claudin-4/CLDN4 Protein(Nanodisc, AVI & His Tag),Biotinylated（产品说明）
蛋白名称：Human Claudin-4/CLDN4 Protein (Nanodisc, AVI & His Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human CLDN4 (O14493) (Ala2-Val209) was expressed with an AVI tag at the N-terminus and a polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells
蛋白纯度：≥ 80 % as determined by SDS-PAGE.
蛋白活性：Immobilized  Recombinant Human Claudin-4/CLDN4 Protein(Nanodisc, AVI & His Tag),Biotinylated  (Cat:16358-H73H-NA-B) at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-Claudin-4 Antibody,human IgG1, the EC50 is 1-5 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met
蛋白分子量：The recombinant Human CLDN4 comprises 238 amino acids and has a predicted molecular mass of 25.52 kDa. It migrates as an approximately 23.4 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：O14493
蛋白氨基酸序列：Ala2-Val209