重组人 CLDN18-Nanodisc (Full Length) 蛋白 (His & Strep 标签)（产品说明）
蛋白名称：Recombinant Human CLDN18-Nanodisc (Full Length) Protein (His & Strep Tag)
蛋白构建：A DNA sequence encoding the Human CLDN18-Nanodisc (Full Length) (P56856-2) (Met1-Val261) was expressed, with a polyhistidine tag at the N-terminus and a Strep tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells
蛋白纯度：≥ 75 % as determined by SDS-PAGE.
蛋白活性：1.Loaded Anti-Human Claudin-18.2 antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human Claudin-18.2-Nanodisc (Full Length) Protein, His & Strep Tag(Cat. No. 30025-H64H-NA) with an affinity constant of 3.43 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 2.Immobilized Recombinant Human CLDN18.2 -Nanodisc (Full  Length) Protein(His & Strep Tag)(Cat:30025-H64H-NA) at 5 μg/mL (100 μL/well) can bind Anti-Human claudin18.2 Antibody, Human IgG1, the EC50 is 4-12 ng/mL.
蛋白内毒素：Please contact us for more information.
预测N端：His
蛋白分子量：The recombinant Human CLDN18-Nanodisc consists of 303 amino acids and predicts a molecular mass of 32.34 kDa. It migrates as an approximately 27.5 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P56856-2
蛋白氨基酸序列：Met1-Val261
蛋白标签：N-His & C-Strep
蛋白保存条件：-20℃ to -80℃