重组人 E-Cadherin / CDH1 / E-cad / CD324 蛋白 (ECD, His标签)（产品说明）
蛋白名称：Human E-Cadherin / CDH1 / E-cad / CD324 Protein (ECD, His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of human E-Cad precusor (NP_004351.1) (Met 1-Ile 707) was fused with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), approximately 20%-40% of cells will adhere specifically after 90 minutes at 37 ℃.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 23 & Asp 155
蛋白分子量：The recombinant pro form of human E-Cad consists of 696 amino acids and has a calculated molecular mass of 77 kDa. As a result of glycosylation, the apparent molecular mass of rh E-Cad is approximately 90.6 kDa corresponding to the mature form and pro form respectively in SDS-PAGE under reducing conditions.
蛋白NP号：NP_004351.1
蛋白氨基酸序列：Met1-Ile707
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.