图文详情
CD9重组蛋白|Recombinant Human CD9 Protein (Nanodisc, Full-length, His-GFP Tag)(产品说明)
蛋白名称:Human CD9 Protein (Nanodisc, Full-length, His-GFP Tag), Fluorescent
蛋白构建:A DNA sequence encoding the Human CD9 (P21926) (Met1-Val228) was expressed with a N-terminal polyhistidine tag followed by a GFP Tag. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
蛋白纯度:≥ 80 % as determined by SDS-PAGE.
蛋白活性:Immobilized Recombinant Fluorescent Human CD9-Nanodisc Protein (Cat: 11029-H87H-NA) at 5 μg/mL (100 μL/well) can bind Anti-CD9 Monoclonal Antibody (Cat: 11029-MM01), the EC50 is 4-30 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Met
蛋白分子量:The recombinant Human CD9 consists of 477 amino acids and predicts a molecular mass of 53.68 kDa. It migrates as an approximately 51.3 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:P21926
蛋白氨基酸序列:Met1-Val228
蛋白标签:N-His-GFP