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重组人CD79B蛋白(PE定点偶联, 胞外区, His标签)(产品说明)
蛋白名称:Human CD79B Protein (Site-Specific PE Conjugation, ECD, His Tag)
蛋白构建:A DNA sequence encoding the human CD79B (NP_000617.1) (Met 1-Asp 159) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with PE (Excitation = 495-566 nm, Emission Max.= 575 nm).
表达宿主:HEK293 Cells
蛋白活性:1.Flow cytometric analysis of anti-CD79B CAR expression. 5e5 of anti-CD79B CAR-293 cells were stained with 100 μL of 1:100 (1 μL stock solution in 100 μL FACS buffer) of PE-conjugated human CD79B Protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 29750-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left). PE signal was used to evaluate the binding activity (QC tested).
2.Binding activity of PE-conjugated CD79B protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human CD79B Protein (Site-Specific PE Conjugation, ECD, His Tag, Cat. No. 29750-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素:Please contact us for more information.Ala 29
蛋白分子量:The protein has a predicted molecular mass of 18.5 kDa.
蛋白NP号:NP_000617.1
蛋白氨基酸序列:Met1-Asp159