重组人 CD47 蛋白 (ECD,Fc标签)（产品说明）
蛋白名称：Human CD47 Protein (ECD,Fc Tag)
蛋白构建：A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His (Cat:11612-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His (Cat:50956-M08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.05-0.13 μg/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His (Cat:11828-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 19
蛋白分子量：The recombinant human CD47/Fc is a disulfide-linked homodimer. The reduced monomer comprises 362 amino acids and has a predicted molecular mass of 40.7 kDa. The apparent molecular mass of the protein is approximately 48-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：NP_942088.1
蛋白氨基酸序列：Met1-Pro139
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.