重组人 CD3D & CD3E Heterodimer 蛋白 (Fc Tag)（产品说明）
蛋白名称：Human CD3D & CD3E Heterodimer Protein (Fc Tag)
蛋白构建：A DNA sequence encoding the Human CD3D (P04234-1) (Met1-Ala105) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the Human CD3E (P07766-1) (Met1-Asp126) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Human CD3D & CD3E Heterodimer was purified.
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested).
蛋白活性：Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein (Fc Tag)(Cat: CT038-H02H) at 1 μg/mL (100 μL/well) can bind Anti-CD3 (OKT3) (Research Grade Muromonab Biosimilar), the EC50 is 1.5-4.5 ng/mL(QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Phe 22 & Asp 23
蛋白分子量：The recombinant Human CD3D comprises 968 (316+652) amino acids and predicts a molecular mass of 108.73 (35.42+73.31) kDa. As a result of glycosylation, it migrates as an approximately 46.7 and 41 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P04234-1 & P07766-1
蛋白氨基酸序列：Met1-Ala105 & Met1-Asp126