重组人CD3D & CD3E Heterodimer蛋白（APC定点偶联, Flag & His标签）（产品说明）
蛋白名称：Human CD3D & CD3E Protein (Site-Specific APC Conjugation, Flag & His)
蛋白构建：A DNA sequence encoding the human CD3D (P04234-1) (Met1-Ala105) was fused with a C-terminal Flag tag. Another DNA sequence encoding the human CD3E (P07766-1) (Met1-Asp126) was fused with a C-terminal polyhistidine tag. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).
表达宿主：HEK293 Cells1.Flow cytometric analysis of anti-CD3(OKT3 derived) CAR expression. 5e5 of anti-CD3 CAR-293 cells were stained with 100 μL of 1:50 (2 μL stock solution in 100 μL FACS buffer) of APC-conjugated human CD3D & CD3E Heterodimer Protein (Site-Specific APC Conjugation, Flag & His Tag, Cat. No. CT038-H2586H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested). 2.Binding activity of APC-conjugated CD3D & CD3E protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CD3D & CD3E Heterodimer Protein (Site-Specific APC Conjugation, Flag & His Tag, Cat. No. CT038-H2586H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Phe 22 & Asp 23
蛋白分子量：The protein has a predicted molecular mass of 33.6 (14.4+19.2) kDa.
蛋白NP号：P04234-1 & P07766-1
蛋白氨基酸序列：Met1-Ala105 & Met1-Asp126