图文详情
重组食蟹猴 CD3D&CD3E 异源二聚体蛋白 (Fc-His & Fc-Flag标签)(产品说明)
蛋白名称:Cynomolgus CD3D & CD3E Heterodimer Protein (Fc-His & Fc-Flag Tag)
蛋白构建:A DNA sequence encoding the cynomolugs CD3D (Q95LI8-1)(Met1-Ala105) with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus CD3E (XP_015290838.1) (Met1-Asp117) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the CD3D&CD3E heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Phe 22 & Gln 22
蛋白分子量:The recombinant heterodimer of cynomolgus CD3D&CD3E comprises 679 (334+345) amino acids and has a calculated molecular mass of 76.63 (37.75+ 38.88) KDa. The apparent molecular mass of rhesus CD3D&CD3E heterodimer is approximately (50.99 & 40.83) KDa respectively in SDS-PAGE.
蛋白NP号:Q95LI8-1 & XP_015290838.1
蛋白氨基酸序列:Met1-Ala105 & Met1-Asp117
蛋白标签:C-human IgG1-Fc & His & C-human IgG1-Fc & Flag
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.