CD38重组蛋白|Recombinant Human CD38 Protein (Site-specific AF 647-conjugated, ECD)（产品说明）
蛋白名称：Human CD38 Protein (Site-specific AF 647-conjugated, ECD, His Tag)
蛋白构建：A DNA sequence encoding the human CD38 (NP_001766.2) (Val 43-Ile 300) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).HEK293 Cells
蛋白活性：1.Flow cytometric analysis of anti-CD38 CAR expression. 5e5 of anti-CD38 CAR-293 cells were stained with 100 μL of 2μg/ml of AF 647-conjugated human CD38 protein (Site-Specific AF 647-Conjugation, ECD, His Tag, Cat. No. 10818-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 647 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 647-conjugated CD38 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human CD38 protein (Site-Specific AF 647-Conjugation, ECD, His Tag, Cat. No. 10818-H86H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Val 43
蛋白分子量：The protein has a predicted molecular mass of 33.4 kDa.
蛋白NP号：NP_001766.2
蛋白氨基酸序列：Val43-Ile300