图文详情
CD38重组蛋白|Recombinant Human CD38 Protein (Site-Specific APC Conjugation, His Tag)(产品说明)
蛋白名称:Human CD38 Protein (Site-Specific APC Conjugation, ECD, His Tag)
蛋白构建:A DNA sequence encoding the human CD38 (NP_001766.2) (Val 43-Ile 300) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).HEK293 Cells
蛋白活性:1.Flow cytometric analysis of anti-CD38 CAR expression. 5e5 of anti-CD38 CAR-293 cells were stained with 100 μL of 1:125 dilution of APC-conjugated human CD38 protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 10818-H86H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested).
2.Binding activity of APC-conjugated CD38 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CD38 protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 10818-H86H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Val 43
蛋白分子量:The protein has a predicted molecular mass of 33.4 kDa.
蛋白NP号:NP_001183.2
蛋白氨基酸序列:Met1-Ala54