General Information
| Expression System: |
HEK293 Cells |
| Preparation: |
Recombinant human CD33 (AAH28152.1, Met1-His259) are conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Labeling Rate: |
The PE to protein molar ratio is 0.1-1 |
| Formulation: |
Aqueous solution containing 0.5% BSA and 0.03%Proclin 300 |
| Concentration: |
10 μl/Test, 0.1 mg/ml |
| Form & Shipping: |
Liquid. Shipping at ambient temperature. |
| Storage: |
This reagent is stable for 6 months from date of receipt when stored at 2℃ - 8℃. Protected from prolonged exposure to light. Do not freeze ! |
| Usage: |
The 5×10E5 cells are suspended in 50 μL washing buffer (1 X PBS + 1% BSA), add 10 μL CD33-PE, incubate for 45 min at 4℃ from light, then wash 2 times with wash buffer. Analyze by flow cytometry immediately. |
荧光标记的靶点蛋白
荧光标记的靶点蛋白,可直接用于CAR分析,避免因二抗交叉反应而引起的非特异性背景,并使检测更加高效。义翘神州已开发了多种荧光标记的靶点蛋白,涵盖当前CAR-T研究相关热门靶点,具有蛋白活性高,均一性好、批间一致性高等特点。同时,依托于先进蛋白表达平台,我们也可以提供“一站式”荧光标记蛋白的定制服务。
血液肿瘤热门靶点蛋白流式检测
使用FITC标记BCMA蛋白检测Anti-BCMA-CAR表达阳性率 (货号:10620-H03H-F)
图1:转染293细胞与FITC荧光标记的BCMA蛋白共孵育后,流式检测,未转染的293细胞作对照,CAR阳性细胞占比59.6%。
使用PE标记CD22蛋白检测Anti-CD22-CAR表达阳性率 (货号:11958-H08H-P)
图2:转染T细胞后与PE荧光标记的CD22蛋白共孵育后,流式检测,未转染的T细胞作对照,CAR阳性细胞占比40.1%。
使用FITC标记CD33蛋白检测Anti-CD33-CAR表达阳性率 (货号:12238-HCCH-F)
图3:转染293细胞与FITC荧光标记的C33蛋白共孵育后,流式检测,未转染的293细胞作对照,CAR阳性细胞占比50.5%。
使用FITC标记CD38蛋白检测Anti-CD38-CAR表达阳性率 (货号:10818-H08H-F)
图4:转染293细胞与FITC荧光标记的CD38蛋白共孵育后,流式检测,未转染的293细胞作对照,CAR阳性细胞占比49.1%。
Human CD33 / Siglec-3 Protein (PE conjugated): 别称
Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.