PD-L1/B7-H1重组蛋白|Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human PD-L1 Protein (Fc & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human CD274 (NP_054862.1)(Met1-Thr239) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性：Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 10377-H02H) at 2 μg/mL (100 μl/well) can bind Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10084-H41H-B), the EC50 is 25-75 ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Phe 19
蛋白分子量：The recombinant Human CD274 consisting of 474 amino acids and has a calculated molecular mass of 53.83 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 71.4 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_054862.1
蛋白氨基酸序列：Met1-Thr239
蛋白标签：C-human IgG1-Fc & AVI