图文详情
PD-L1/B7-H1重组蛋白|Recombinant Human PD-L1 / B7-H1 / CD274 Protein (His Tag) , Biotinylated(产品说明)
蛋白名称:Human PD-L1 / B7-H1 / CD274 Protein (His Tag) , Biotinylated
蛋白构建:A DNA sequence encoding the N-terminal segment (Met1-Thr239) of the extracellular domain of human B7-H1 (NP_054862.1) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
表达宿主:HEK293 Cells
蛋白纯度:> 95% as determined by SDS-PAGE.
蛋白活性:Measured by its binding ability in a functional ELISA. Immobilized Human PD-1 His & hFc(Cat:10377-H03H) at 2 μg/ml (100 μl/well) can bind Human PD-L1 His, Biotinylated(Cat:10084-H08H-B), the EC50 of Human PD-L1 His, Biotinylated is 50-300 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Phe 19
蛋白分子量:The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the human B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:NP_054862.1
蛋白氨基酸序列:Met1-Thr239
蛋白标签:C-His