重组人 PD-L1 / B7-H1 / CD274 蛋白 (Fc标签)（产品说明）
蛋白名称：Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag)
蛋白构建：A DNA sequence encoding the N-terminal segment (Met 1-Thr 239) of the extracellular domain of human B7-H1 (NP_054862.1) was expressed with C-terminal fused Fc region of human IgG1.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized human PD-1 (Cat: HPLC-10377-H08H) at 2 μg/ml (100 μl/well) can bind human B7-H1(Cat: 10084-H02H), the EC50 of human B7-H1 is 150-600 ng/mL. 2. Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (Fc Tag)(10084-H02H) bound to Atezolizuma was 0.5 μM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Phe 19
蛋白分子量：The recombinant mature human B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the human B7-H1/Fc monomer migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：NP_054862.1
蛋白氨基酸序列：Met1-Thr239
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.