图文详情
小鼠 CD27蛋白|CD27 protein|CD27(Mouse, C-human IgG1-Fc & His)(产品说明)
蛋白名称:CD27蛋白, CD27 protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Arg 182) of mouse CD27 (NP_001028298.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 94 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml .
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Thr 21
蛋白分子量:The recombinant mouse CD27/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 410 amino acids and has a predicted molecular mass of 46.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD27/Fc monomer is approximately 60-65 kDa due to glycosylation.
蛋白NP号:NP_001028298.1
蛋白氨基酸序列:Met1-Arg182
蛋白标签:C-human IgG1-Fc & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.