Human 2B4 / CD244 Protein (Fc & AVI Tag), Biotinylated（产品说明）
蛋白名称：Human 2B4 / CD244 Protein (Fc & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human CD244 (NP_057466.1) (Met1-Arg221) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：Measured by its binding ability in a functional ELISA. Immobilized Human CD48 hFc(Cat: 10797-H02H) at 2 μg/mL (100 μL/well) can bind Human 2B4/CD244 (hFc & AVI Tag), Biotinylated(Cat: 10042-H41H-B), the EC50 of Human 2B4/CD244 (hFc & AVI Tag), Biotinylated(Cat:10042-H41H-B) is 10-70 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Cys 22
蛋白分子量：The recombinant human CD244 consists of 453 amino acids and predicts a molecular mass of 50.80 kDa. It migrates as an approximately 65.58 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_057466.1
蛋白氨基酸序列：Met1-Arg221
蛋白标签：C-human IgG1-Fc & AVI
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.