CCR8重组蛋白|Recombinant Human CCR8 Protein (Nanodisc, Flag-Strep & His Tag)（产品说明）
蛋白名称：Human CCR8 Protein (Nanodisc, Flag-Strep & His Tag)
蛋白构建：A DNA sequence encoding the Human CCR8 (P51685) (Met1-Cys228 & Asn230-Ser318, thermostabilizing mutations) (TM domains 1-7) was expressed and linked by a Fusion Protein, with a Flag tag followed by a Strep Tag at the N-terminus, a polyhistidine tag at the C-terminus. 〈br/〉Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells
蛋白纯度：≥ 85 % as determined by SDS-PAGE.
蛋白活性：1. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody1, Human IgG1, the  EC50 is 1-5 ng/mL (QC tested). 2. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody2, Human IgG1, the  EC50 is 3-10 ng/mL (Routinely tested). 3. Immobilized Recombinant Human CCR8-Nanodisc Protein (Cat:11450-H91H-NA) at 5 μg/mL (100 μL/well) can bind Anti- Human CCR8 Monoclonal Antibody3, Human IgG1, the  EC50 is 3-10 ng/mL (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 1
蛋白分子量：The recombinant Human CCR8 protein predicts a molecular mass of 46.51 kDa. The recombinant protein migrates as an approximately 96.9 kDa (dimer) and 44 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号：P51685
蛋白氨基酸序列：Met1-Cys228 & Asn230-Ser318
蛋白标签：N-Flag-Strep & C-His