图文详情
carbonic anhydrase XII/CA12重组蛋白|Recombinant Mouse Carbonic Anhydrase XII / Car12 Protein (His Tag)(产品说明)
蛋白名称:Mouse Carbonic Anhydrase XII / Car12 Protein (His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Ser 301) of mouse CA12 (NP_848483.2) precursor was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
蛋白活性:Measured by its esterase activity. The specific activity is >50 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.4 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ala 25
蛋白分子量:The secreted recombinant mouse CA12 consists of 288 amino acids and has a calculated molecular mass of 32.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:NP_848483.2
蛋白氨基酸序列:Met1-Ser301
蛋白标签:C-His