图文详情
重组人 BACE1 / ASP2 蛋白 (His标签)(产品说明)
蛋白名称:Human BACE1 / ASP2 Protein (His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (amino acid residue Met 1-Thr 457) of human BACE1 (NP_036236.1) precursor was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2, R&D Systems, Catalog # ES004. The specific activity is >1.5 pmoles/min/μg.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Thr 22 and Glu 46 (rare)
蛋白分子量:The recombinant human BACE1 consists of 446 amino acids and has a calculated molecular mass of 49.9 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60-65 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:NP_036236.1
蛋白氨基酸序列:Met1-Thr457
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.