图文详情
重组人 Angiopoietin-2 蛋白 (AVI & His 标签), 生物素标记(产品说明)
蛋白名称:Human Angiopoietin-2 Protein (AVI & His Tag), Biotinylated
蛋白构建:A DNA sequence encoding the human Angiopoietin-2 (NP_001138.1) (Lys275-Phe496) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
蛋白活性:Immobilized Recombinant Human Tie2 / CD202b / TEK Protein (ECD, His Tag)(Cat:10700-H08H) at 2 μg/ml (100 μl/well) can bind Recombinant Human Angiopoietin-2 Protein (AVI & His Tag), Biotinylated(Cat:10691-H27H1-B), the EC50 is 300-900 ng/mL.
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Lys 275
蛋白分子量:The recombinant human ANGPT2 consists of 248 amino acids and predicts a molecular mass of 28.72 kDa. It migrates as an approximately 30.74 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001138.1
蛋白氨基酸序列:Lys275-Phe496
蛋白标签:C-AVI & His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.