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小鼠 ACVR2B蛋白|ACVR2B protein|ACVR2B(Mouse, C-His)(产品说明)
蛋白名称:ACVR2B蛋白, ACVR2B protein
蛋白构建:A DNA sequence encoding the extracellular domain of mouse ACTRIIB (NP_031423.1) (Met 1-Thr 134) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 94 % as determined by SDS-PAGE
蛋白活性:1. Measured by its binding ability in a functional ELISA. Immobilized human ACVR2B (Cat:10229-H02H) at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His (Cat:50659-M08H), The EC50 of biotinylated mouse INHBA-His (Cat:50659-M08H) is 0.161 μg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse INHBA-his at 10 μg/mL (100 μl/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is 0.39 μg/mL. 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.2-0.8 µg/mL in the presence of 10 ng/mL recombinant Activin A.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser l9
蛋白分子量:The recombinant mouse ACTRIIB/ACVR2B comprises 127 amino acids and has a predicted molecular mass of 14.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B is approximately 33-37 kDa due to glycosylation.
蛋白NP号:NP_031423.1
蛋白氨基酸序列:Met1-Thr134
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.