A2AR重组蛋白|Recombinant Human A2aR-Nanodisc Protein(His&Flag Tag,thermostabilizing mutations)（产品说明）
蛋白名称：Human A2aR-Nanodisc Protein (His & Flag Tag, thermostabilizing mutations)
蛋白构建：A DNA sequence encoding the human AA2AR (P29274) (Met1-Ala316, thermostabilizing mutations) was expressed, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus.Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE.
蛋白活性：Immobilized Human ADORA2A-SMA Nanodisc Protein, (Cat: 12307-H18H1-NA) at 5 μg/mL (100 μL/well) can bind Anti-Human ADORA2A Monoclonal antibody, Mouse IgG2a,the EC50 is 3-9ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Asp
蛋白分子量：The recombinant human AA2AR predicts a molecular mass of 37.25 kDa. The apparent molecular mass of the protein is approximately 33-37 kDa (due to glycosylation) in SDS-PAGE under reducing conditions.
蛋白NP号：P29274
蛋白氨基酸序列：Met1-Ala316
蛋白标签：N-Flag & C-His