图文详情
A2AR重组蛋白|Recombinant Human A2aR -Nanodisc Protein(His & Flag Tag)(产品说明)
蛋白名称:Human A2aR -Nanodisc Protein(His & Flag Tag)
蛋白构建:A DNA sequence encoding the human AA2AR (P29274) (Pro2-Leu208 & Glu219-Ala316) (TM domains 1-7) was expressed and linked by a Fusion Protein, with a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主:HEK293 Cells
蛋白纯度:≥ 95 % as determined by SDS-PAGE.
蛋白活性:1.Immobilized Recombinant Human ADORA2A-Nanodisc Protein (Cat: 12307-H18H-NA) at 5 μg/mL (100 μL/well) can bind Anti-HumanADORA2A Monoclonal Antibody, Mouse IgG2a, the EC50 is 2-6 ng/mL. 2.Loaded Human A2aR -Nanodisc Protein, His & Flag Tag (Cat. No. 12307-H18H-NA) on AR2G Biosensor via Anti-Fusion Protein Monoclonal antibody, Mouse IgG2a, can bind Anti-Human ADORA2A Monoclonal antibody, Mouse IgG2a with an affinity constant of 0.847nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Loaded Anti-Human ADORA2A Monoclonal antibody, Mouse IgG2a on ProA Biosensor, can bind Human A2aR -Nanodisc Protein, His & Flag Tag (Cat. No. 12307-H18H-NA) with an affinity constant of 2.34 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Asp
蛋白分子量:The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions. FP6 predicts a molecular mass of 11.83 kDa
蛋白NP号:P29274
蛋白氨基酸序列:Pro2-Leu208 & Glu219-Ala316
蛋白标签:N-Flag & C-His