SARS-CoV-2 (2019-nCoV) 3CLpro / 3C-like protease-His & AVI Recombinant Protein: 产品信息
纯度> 90 % as determined by SDS-PAGE.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Measured by its ability to cleave a peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans.The specific activity is >3 pmols/min/μg
蛋白构建A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) 3CLpro (YP_009725295.1) (Ser3264-Gln3569) was expressed with a N-terminus polyhistide tag and AVI tag at the N-terminus.
NP No.YP_009725295.1
表达宿主Baculovirus-Insect Cells
种属SARS-CoV-2
预测 N 端Met
分子量The recombinant heterodimer of SARS-CoV-2 (2019-nCoV) 3CLpro consists of 332 amino acids and has a calculated molecular mass of 37.12 KDa.
缓冲液Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.