图文详情
重组人IL-35蛋白(产品说明)
蛋白名称:Human IL-35 (IL12A & EBI3 Heterodimer) Protein, Endotoxin-Free
蛋白构建:A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a C-terminal Fc region of human IgG1 tag followed by a His tag, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE.
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Arg 23 & Arg 21
蛋白分子量:The recombinant human IL12A&IL27B comprises 905 (447+458) amino acids and has a calculated molecular mass of 102 (50.7+ 51.3) KDa.
蛋白NP号:P29459-1 & NP_005746.2
蛋白氨基酸序列:Met1-Ser219 & Met1-Lys229