重组人 DLL3蛋白 (APC定点偶联, His标签)（产品说明）
蛋白名称：Human DLL3 Protein (Site-Specific APC Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the Human DLL3 (Q9NYJ7-1) (Ala 27-Leu 492) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with APC (Excitation Max.= 651 nm, Emission Max.= 662 nm).
表达宿主：HEK293 Cells
蛋白纯度：
蛋白活性：1.Flow cytometric analysis of anti-DLL3 CAR expression. 5e5 of anti-DLL3 CAR-293 cells were stained with 100 μL of 1:50 (2 μL stock solution in 100 μL FACS buffer) of APC-conjugated human DLL3 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 20010-H86H5-SA) and negative control protein. Non-transduced 293 cells were used as a control (left). APC signal was used to evaluate the binding activity (QC tested). 2.Binding activity of APC-conjugated DLL3 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human DLL3 Protein (Site-Specific APC Conjugation, His Tag, Cat. No. 20010-H86H5-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Ala 27
蛋白分子量：The protein has a predicted molecular mass of 52.3 kDa.
蛋白NP号：Q9NYJ7-1
蛋白氨基酸序列：Ala27-Leu492